Cofilin antibody (AA 1-5)

Catalog No. ABIN363152

The Rabbit Polyclonal anti-Cofilin antibody has been validated for WB and IHC. It is suitable to detect Cofilin in samples from Human, Mouse and Rat. There is 1 publication available.

Quick Overview for Cofilin antibody (AA 1-5) (ABIN363152)

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cofilin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Cofilin Antibody

Binding Specificity: AA 1-5

Specificity: The antibody detects endogenous level of total cofilin protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Peptide sequence around AA 1-5 (M-A-S-G-V) derived from Human cofilin. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-Cofilin antibody (ABIN363152)

Zhang, Luo, Wan, Wu, Laski, Chen: "Regulation of cofilin phosphorylation and asymmetry in collective cell migration during morphogenesis." in: Development (Cambridge, England), Vol. 138, Issue 3, pp. 455-64, (2011) (PubMed).

Target Details for Cofilin

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Alternative Name: cofilin

Background: Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.

Molecular Weight: 19 kDa

NCBI Accession: NP_000213

UniProt: P23528

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events